A Novel Form of RNA Polymerase from Escherichia coli
نویسندگان
چکیده
منابع مشابه
A novel form of RNA polymerase from Escherichia coli.
A new form of RNA polymerase, termed RNA polymerase III, has been recognized as a large fraction of the rifampicin-sensitive enzyme in E. coli. It is physically separable from RNA polymerase (holoenzyme, RNA polymerase I) by gel filtration and is distinguished by its capacity to discriminate between M13 and varphiX174 viral DNA templates in priming DNA synthesis. This template specificity is ma...
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A new form of RNA polymerase, termed RNA polymerase 111, has been recognized as a large fraction of the rifampicin-sensitive enzyme in E. coli. It is physically separable from RNA polymerase (holoenzyme, RNA polymerase I) by gel filtration and is distinguished by its capacity to discriminate between M13 and 4x174 viral DNA templates in priming DNA synthesis. This template specificity is manifes...
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Escherichia coli RNA polymerase exists in two different forms, one less active than the other under standard assay conditions. Conversion of the active to the inactive form requires ATP-Mg(++). The reverse, inactive to active enzyme conversion, requires Mg(++). Covalently bound adenylic acid residue(s) are contained in the inactive form.
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Noncoding RNAs (ncRNAs) regulate gene expression in all organisms. Bacterial 6S RNAs globally regulate transcription by binding RNA polymerase (RNAP) holoenzyme and competing with promoter DNA. Escherichia coli (Eco) 6S RNA interacts specifically with the housekeeping σ70-holoenzyme (Eσ70) and plays a key role in the transcriptional reprogramming upon shifts between exponential and stationary p...
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To obtain an estimate for the concentration of free functional RNA polymerase in the bacterial cytoplasm, the content of RNA polymerase beta and beta' subunits in DNA-free minicells from the minicell-producing Escherichia coli strain chi925 was determined. In bacteria grown in Luria-Bertani medium at 2.5 doublings/h, 1.0% of the total protein was RNA polymerase. The concentration of cytoplasmic...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.11.4425